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Genetic engineering : principles and advancements / edited by Vikas Mishra.

Contributor(s): Material type: TextPublication details: Oakville, Ontario : Delve Publishing, c2019Description: xxvii, 396 pages : color illustrationsContent type:
  • text
Media type:
  • computer
Carrier type:
  • online resource
Subject(s): LOC classification:
  • EBOP QH 442 G46 2019
Contents:
SECTION I GENETIC ENGINEERING IN ANIMALS Chapter 1: Birth of Clones of The World’s First Cloned Dog Chapter 2: Generation of Cloned Mice and Nuclear Transfer Embryonic Stem Cell Lines From Urine-Derived Cells Chapter 3: A One-Step Cloning Method For The Construction of Somatic Cell Gene Targeting Vectors: Application to Production of Human Knockout Cell Lines Chapter 4: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination SECTION II GENETIC ENGINEERING IN PLANTS Chapter 5: A Modified Gateway Cloning Strategy for Overexpressing Tagged Proteins in Plants Chapter 6: A Simple, Flexible and Efficient PCR-Fusion/Gateway Cloning Procedure For Gene Fusion, Site-Directed Mutagenesis, Short Sequence Insertion and Domain Deletions and Swaps Chapter 7: Colorful-Circuit: A Platform For Rapid Multigene Assembly, Delivery, And Expression In Plants Chapter 8: CRISPR-Based Tools For Plant Genome Engineering SECTION III GENETIC ENGINEERING IN DROSOPHILA Chapter 9: Single Vector Non-Leaky Gene Expression System For Drosophila Melanogaster Chapter 10: Systematic Comparison of 2A Peptides For Cloning Multi-Genes in a Polycistronic Vector Chapter 11: An Entry/Gateway® Cloning System For General Expression of Genes With Molecular Tags In Drosophila Melanogaster Chapter 12: Gene Tagging Strategies To Assess Protein Expression, Localization, And Function In Drosophila Chapter 13: Damage To The Drosophila Follicle Cell Epithelium Produces “False Clones” With Apparent Polarity Phenotypes Chapter 14: Dm5-HT2B: Pharmacological Characterization Of The Fifth Serotonin Receptor Subtype Of Drosophila Melanogaster
Summary: Genetic Engineering: Principles and Advancements considers various aspects of genetic engineering including an extensive overview of genetic engineering and related principles and advancements. It includes Molecular Biology and Biotechnology Birth of clones of the world’s first cloned dog, generation of cloned mice and nuclear transfer embryonic stem cell lines from urine-derived cells, a one-step cloning method for the construction of somatic cell gene targeting vectors: application to production of human knockout cell lines. Provides the reader with insights into the development of its history, so as to understand the Dm5-HT2B: Pharmacological Characterization of the Fifth Serotonin Receptor Subtype of Drosophila melanogaster, Extensive cross-regulation of post-transcriptional regulatory networks in Drosophila.
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SECTION I GENETIC ENGINEERING IN ANIMALS
Chapter 1: Birth of Clones of The World’s First Cloned Dog
Chapter 2: Generation of Cloned Mice and Nuclear Transfer Embryonic Stem Cell Lines From Urine-Derived Cells
Chapter 3: A One-Step Cloning Method For The Construction of Somatic Cell Gene Targeting Vectors: Application to Production of Human Knockout Cell Lines
Chapter 4: Cloning, Assembly, and Modification of the Primary Human Cytomegalovirus Isolate Toledo by Yeast-Based Transformation-Associated Recombination
SECTION II GENETIC ENGINEERING IN PLANTS
Chapter 5: A Modified Gateway Cloning Strategy for Overexpressing Tagged Proteins in Plants
Chapter 6: A Simple, Flexible and Efficient PCR-Fusion/Gateway Cloning Procedure For Gene Fusion, Site-Directed Mutagenesis, Short Sequence Insertion and Domain Deletions and Swaps
Chapter 7: Colorful-Circuit: A Platform For Rapid Multigene Assembly, Delivery, And Expression In Plants
Chapter 8: CRISPR-Based Tools For Plant Genome Engineering
SECTION III GENETIC ENGINEERING IN DROSOPHILA
Chapter 9: Single Vector Non-Leaky Gene Expression System For Drosophila Melanogaster
Chapter 10: Systematic Comparison of 2A Peptides For Cloning Multi-Genes in a Polycistronic Vector
Chapter 11: An Entry/Gateway® Cloning System For General Expression of Genes With Molecular Tags In Drosophila Melanogaster
Chapter 12: Gene Tagging Strategies To Assess Protein Expression, Localization, And Function In Drosophila
Chapter 13: Damage To The Drosophila Follicle Cell Epithelium Produces “False Clones” With Apparent Polarity Phenotypes
Chapter 14: Dm5-HT2B: Pharmacological Characterization Of The Fifth Serotonin Receptor Subtype Of Drosophila Melanogaster

Genetic Engineering: Principles and Advancements considers various aspects of genetic engineering including
an extensive overview of genetic engineering and related principles and advancements. It includes Molecular Biology
and Biotechnology Birth of clones of the world’s first cloned dog, generation of cloned mice and nuclear transfer
embryonic stem cell lines from urine-derived cells, a one-step cloning method for the construction of somatic cell gene
targeting vectors: application to production of human knockout cell lines. Provides the reader with insights into the
development of its history, so as to understand the Dm5-HT2B: Pharmacological Characterization of the Fifth Serotonin
Receptor Subtype of Drosophila melanogaster, Extensive cross-regulation of post-transcriptional regulatory networks
in Drosophila.

EBOP00027 1

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